Thilini Abeywansha

4 Questions 3 Answers 0 Followers

Questions related from Thilini Abeywansha

Methods to count copy number of a specific protein per cell ?

I need to find the number of copies of a specific membrane protein per E. Coli cell . I've created different mutations of this proteins and I need to figure out how would these mutations affect...

01 July 2020 9,138 0 View

What would be a critical gene to remove to prevent ATP synthesis from F1F0 ATP synthase in E.Coli ?

I need to prevent ATP production from F1F0 ATP synthase in E.Coli for an control experiment. What would be a critical gene to remove to delete F1F0 synthase completely or prevent it from...

04 December 2019 3,886 2 View

Chloramphenicol Acetyltransferase assay in E.Coli ?

Has anyone tried this assay with E.Coli cells ? I would like to know the ideas and experiences . Thank you. A Rapid, Sensitive, and Inexpensive Assay for Chloramphenico...

23 July 2019 4,795 0 View

After Sonication and centrifugation of bacterial cells, in which fraction is periplasmic materials exist? in the supernatent or the pellet ?

After Sonication and centrifugation of bacterial cells, in which fraction is the periplasmic materials exist? in the supernatent or the pellet ?

04 June 2019 3,732 4 View