Hi, could you give me an advice about chromatin immunoprecipitation in bacteria? Is it possible to use DNase for removal nonbinding part of DNA? I mean this: protein will be bind on part of digested DNA, you will use DNase for removal part of DNA, which doesn´t bind my protein. Theoretically, it will remain only concrete part of DNA that bind my protein, because the protein protect phosphodiester bound. What do you think?
Lee Siggens
I think what you are looking for is a ChIP-exo protocol. I wouldn't use DNase but ChIP-exo is basically doing what you are asking for. for example see .... https://www.ncbi.nlm.nih.gov/pubmed/23026909
p.s. do bacteria have chromatin? :)
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Pavla Pavlik
Thank you very much :-) I will read up it.
btw, chromatin - you know what I mean :-)
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