What software do you use for qualitative content analysis of articles (for free)?

More Pavla Vrabcová's questions See All

DNA shearing by french press?

Is it possible to shear DNA by french press to get fragment size about 500 bp?

08 October 2017 1,014 12 View

Prediction tool for DNA-binding site?

Hi, could you recommended me any prediction software for DNA-binding sites of my protein? I have protein with predict structure using Psipred (pdb file) and fasta file with genome. I would like to...

26 July 2017 7,171 3 View

What experiences with Primocin do you have?

Hi everybody, I'm treating my contaminated cell cultures by primocin. What experiences do you have? What is the optimal length of treatment to obtain healthy cell culture? Do you use primocin all...

15 May 2017 1,440 2 View

Denaturing PAGE of DNA - how to get sharp bands?

Our research group has recetly bought CBS Scientific Adjustable Sequencing Kit (SG-400-20) and we are facing troubles with our electrophoretic separations because we are unable to obtain sharp...

26 February 2017 4,223 3 View

How to balance ChIP-seq library prep for multiplex sequencing?

I am preparing ChIP-seq libraries for Illumina multiplexing. I have large amount of the ChIP-input DNA compared to the ChIP-output. I wonder if it is better to keep the DNA amount for library...

16 August 2016 1,075 4 View

RNA immunoprecipitation - why Western blot doesn't work but RT-PCR does?

Hi everyone! I try to optimize immunoprecipitation method followed by RNA isolation and RT-PCR/real-time. I did it for a first time and I did not have any experience with this. I managed to...

02 August 2016 2,013 10 View

Has anyone experience with ChIP and bacterial transcriptional factors?

I am going to try this method for prokaryotic cells - gram negative bacteria. I want to define regulon of DNA-binding protein. My protein is non specific binding to dsDNA. Is it a problem?

07 February 2016 7,323 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Alfred Howard Miller

KH Coder which I mentioned before on RG, is open source, has an active user community and a responsive author. I ran into difficulty deploying a particularly large data set for analysis and Ko-icthi Higuchi helped me with sampling in KH Coder, which is a form of bootstrapping, and assured me of its validity. Ultimately, with his advice, I was able to get my rather large sample to run.