I would like to design a few couples of primers to amplify several define transcript region of IgG gene. With forward primers in the V or J region and reverse Primers in the constant region. However, I can not check the primer specificity using NCBI primer blast. Do you know which platform or sofware that I can do primer blast for IgG transcript? Thanks and regards.
Frederic Lepretre
Hi Thuy
you can check on the UCSC database, there is a tool named "in-silico PCR", but if it's for human, the issue is well documented in papers since of huge variability in this region, degenerated primers are often used. on UCSC, in hg19, you can also check for qPCR primers designed in former studies (optional track in the expression toolbar).
all the best
fred
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