I am modifying graphene with amino linked aptamer with PBASE crosslinker. Can you please let me know whether it is possible to characterize aptamer immoblization on graphene using XPS?
Dear,
let's say If you targeted -O- or -COOH and any kind of functional group, one of the best ever invented method for this purpose is FTIR. I know this is a bit complicated because I have involved with this problem, by the way, both chemically or physically immobilization could be tracked with IR.
XPS is more like to be detour from the real answer. Go the long and difficult pathway to find out more ;)))))))
Dear,
let's say If you targeted -O- or -COOH and any kind of functional group, one of the best ever invented method for this purpose is FTIR. I know this is a bit complicated because I have involved with this problem, by the way, both chemically or physically immobilization could be tracked with IR.
XPS is more like to be detour from the real answer. Go the long and difficult pathway to find out more ;)))))))
As @Amir mentioned, XPS is not that straightforward for solving your problem. You'll get pretty much the same XPS peaks for oxiygen in different coordination environments. So a differentiation will be difficult. You may try to get "fingerprints" for different species, nevertheless I agree that other methods are more direct (specific) for your actual problem. In any case, however, if you have an XPS system available in your lab, I would go and try out! Good luck, Dirk
Agreed with Amir and Dirk. XPS might not be the straight forward method. You can characterize the aptamer immobilization by electrochemical techniques such as EIS, DPV or SWV
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