Dear Shamsul,

The following is the procedure of the palmitation taken from two different sources:

1-Recombinant protein G (Pierce, Rockford, IL) was derivatized with N-hydroxysuccinimide ester of palmitic acid (Sigma, St. Louis, MO), as previously described [8] except the palmitation reaction was conducted at 4°C for 1.5 hr instead of at 37°C. The lipid-derivatized protein G was purified on a 10 ml Sephadex G-25 (Pharmacia, Piscataway, NJ) column as described previously [29].

[8]. Dennis JE, Cohen N, Goldberg VM, Caplan AI. Targeted delivery of progenitor cells for cartilage repair. J Orthop Res. 2004;22:735–41. [PubMed]

[29]. Huang A, Huang L, Kennel SJ. Monoclonal antibody covalently coupled with fatty acid. A reagent for in vitro liposome targeting. J Biol Chem. 1980;255:8015–8. [PubMed]

Source: Biomaterials. Author manuscript; available in PMC 2010 Aug 1.

Published in final edited form as:

Biomaterials. 2009 Aug; 30(22): 3702–3710.

Published online 2009 Apr 17. doi: 10.1016/j.biomaterials.2009.03.038

PMCID: PMC2693266

NIHMSID: NIHMS106547

Targeting mesenchymal stem cells to activated endothelial cells

In Kap Ko,1 Thomas J. Kean,1 and James E. Dennis1,*

2-Palmitated protein G (PPG) was derivitized as described previously by reacting recombinant protein G (Pierce, Rockford, IL) and N-hydroxysuccinimide ester of palmitic acid (Sigma, St. Louis, MO) followed by purification using Sephadex G-25 (Pharmacia, Piscataway, NJ).

www.nature.com/mt/journal/v18/n7/extref/mt201054x8.doc

Hoping this will be helpful,

Rafik

Hello

Also, look at these papers may help you

Good Luck 

http://www.jbc.org/content/273/25/15830.full.html

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2693266/

http://www.orangecountyhairrestoration.org/webdocuments/ADSC-hair-restoration.pdfhttp://www.biotechniques.com/multimedia/archive/00036/BTN_A_04362RR02_O_36231a.pdf

Thanks for the info guys