Cell culture 3T3-L1 adipocytes. I can grown in DMEM medium with or without pyruvate pyruvate?

More Orlando Mamani Calle's questions See All

What is the best medium culture for hepatocytes?

Hello dears,I will thank you for your answers, I want to culture hepatocyte in Williams medium E, but I am not sure how I do. after the culture how I can resuspend this cells. Do you have a...

01 February 2017 1,676 4 View

I'm isolating islets of Swiss albino mouse, Without obtaining whole islets. can you help me?

I'm isolating islets of Swiss albino mouse, no problem inflating collagenase solution into the pancreas through the bile duct. Without obtaining whole islets Type V collagenase Some suggestions...

03 April 2016 8,546 0 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Alessio Reggio

Hi, Orlando.

Usually the growth medium for 3T3-L1 is composed by DMEM F-12+ 1%P/S.

Sibtain Afzal

Hi Orlando

Although there are several preadipocyte cell lines reported, I am going to send you a protocol from a book that describe mainly the 3T3-L1 cells, which are best characterized and widely used for molecular biological studies.

I hope it will help you out

Best Regards

Dear Orlando

here is anotherbook which might be very useful for you. its a gift for u.

best regards

Orlando Mamani Calle

Thank you so much Antonio Ruiz Vela, Alessio Reggio and Sibtain Afzal, I appreciate your cooperation

Qixiang ma

Hi,I also want to know the answer