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Questions related from Qixiang ma
I need to KO a gene in a stem cell lines cultured in medium ,However,transect and pick up clones is difficult. So I use Lenticrispr which express lentivirus to KO. under the circumstances, How to...
07 July 2019 930 3 View
Copy from a paper : ‐Make 100 mM stock of: -‐ 25.5 ml 1 M Na2HPO4 -‐74.5 ml 1 M NaH2PO4 -‐pH to 6.4 with NaOH But can I adjust pH with NaOH?I thought that mix appropriate volumes of both...
10 October 2016 3,122 6 View
I'm culturing the RXF393 cell line,gift from a friend.But I find it has two appearance.I wonder that it may be contaminated.Does any one has one and send me a picture of the cell line?
09 September 2016 5,675 2 View
I'm going to study the CRISPR/Cas9 konckout technology .Is there a book that gives a detail on the principle and theory?
08 August 2016 3,602 1 View
When we use the luciferase as a reporter of the tumor volume in mice,it cayalyze luciferin to produce light.But after then ,does luciferase need to be re-activated to catalyze another round of...
07 July 2016 5,192 1 View
