I'm isolating islets of Swiss albino mouse, Without obtaining whole islets. can you help me?

Orlando Mamani Calle @Orlando_Mamani_Calle

04 April 2016 0 9K Report

I'm isolating islets of Swiss albino mouse, no problem inflating collagenase solution into the pancreas through the bile duct.

Without obtaining whole islets

Type V collagenase Some suggestions please protocol use at a concentration of 0.3 - 0.5 mg / ml. Incubation time 10 to 37 ° C. It seems that Swiss albino pancreatic tissue is very delicate in relation to other mice.

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