How to detect transmembrane anti-CD3 antibody?

Hi, I designed a construct encoding extracelluar domain of anti-CD3 antibody and transmembrane domain of CD8. Does anyone have any experience of detect membrane anchored anti-CD3 antibody? what...

07 August 2017 5,863 1 View

How to produce vaccinia virus in large scale for mouse model injectin?

I need to inject 5X10^7pfu vaccinia virus into one mouse. So I have to produce more than 10^9 pfu vaccinia virus. Now I can only produce 10^8 pfu (1ml) virus from 10X150mm tissue plates using...

08 September 2016 922 1 View

Who has A431 cell line?

Who has A431 cell line? I want to use it as EGFR positive control. Can someone borrow me one frozen vial of A431 cell line? We can discuss privately if you have. Thank you

01 February 2016 9,872 0 View

Which cell line is best for PD1 silence experiment?

I want to know if my PD1 siRNA works. I don't know which T cell line is suitable which generates detectable intrinsic PD1. Since T cell lines are suspension cells which is hard to be transfected...

01 February 2016 1,648 2 View

How to test siRNA or shRNA silence efficiency toward PD-1?

I want to test siRNA or shRNA silence efficiency toward PD-1 in whatever cell lines, just to make sure that our siRNA sequence works. Which way is the easiest, WB, FACS or qRTPCR? which cell line...

01 February 2016 3,759 1 View

Is there any commercial anti-EGFRvIII antibody for flow cytometry recommendation?

I want to test the EGFRvIII expression in my cell line. I am wondering if there is any commercial anti-EGFRvIII antibody for flow cytometry? Has anyone have this experience?

31 December 2015 3,866 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View