I am wondering if there is commercial CD8+ T celll line available. Any recommendation? I want to test cell killing assay.
If you are looking for a human cell line you could try TALL-104 cell line. Check its characteristics at ATCC website.
http://www.atcc.org/products/all/CRL-11386.aspx#characteristics
Can you push this line toward exhaustion and then demonstrate recovery?
I have tried unsuccessfully to culture the TALL-104 cell line twice now, following the ATCC instructions exactly. Does anyone know of an alternative (or even a stable cell line that expresses human CD8)?
Sheena N. Smith, same happened to me.
I am also looking for an alternative
Hi guys, do I understand you correctly? You are looking for any human CD8 T cell line that is able to kill any target cell?
Dinesh Adhikary, yes. If you know of any cell-line other than TALL-104, this would be great.
Hi, I designed a construct encoding extracelluar domain of anti-CD3 antibody and transmembrane domain of CD8. Does anyone have any experience of detect membrane anchored anti-CD3 antibody? what...
07 August 2017 5,918 1 View
I need to inject 5X10^7pfu vaccinia virus into one mouse. So I have to produce more than 10^9 pfu vaccinia virus. Now I can only produce 10^8 pfu (1ml) virus from 10X150mm tissue plates using...
08 September 2016 1,000 1 View
Who has A431 cell line? I want to use it as EGFR positive control. Can someone borrow me one frozen vial of A431 cell line? We can discuss privately if you have. Thank you
01 February 2016 9,913 0 View
I want to know if my PD1 siRNA works. I don't know which T cell line is suitable which generates detectable intrinsic PD1. Since T cell lines are suspension cells which is hard to be transfected...
01 February 2016 1,704 2 View
I want to test siRNA or shRNA silence efficiency toward PD-1 in whatever cell lines, just to make sure that our siRNA sequence works. Which way is the easiest, WB, FACS or qRTPCR? which cell line...
01 February 2016 3,807 1 View
I want to test the EGFRvIII expression in my cell line. I am wondering if there is any commercial anti-EGFRvIII antibody for flow cytometry? Has anyone have this experience?
31 December 2015 3,925 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View