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Questions related from Guan Wang
Hi, I designed a construct encoding extracelluar domain of anti-CD3 antibody and transmembrane domain of CD8. Does anyone have any experience of detect membrane anchored anti-CD3 antibody? what...
08 August 2017 5,902 1 View
I need to inject 5X10^7pfu vaccinia virus into one mouse. So I have to produce more than 10^9 pfu vaccinia virus. Now I can only produce 10^8 pfu (1ml) virus from 10X150mm tissue plates using...
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I want to test siRNA or shRNA silence efficiency toward PD-1 in whatever cell lines, just to make sure that our siRNA sequence works. Which way is the easiest, WB, FACS or qRTPCR? which cell line...
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Who has A431 cell line? I want to use it as EGFR positive control. Can someone borrow me one frozen vial of A431 cell line? We can discuss privately if you have. Thank you
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I want to know if my PD1 siRNA works. I don't know which T cell line is suitable which generates detectable intrinsic PD1. Since T cell lines are suspension cells which is hard to be transfected...
02 February 2016 1,688 2 View
I am wondering if there is commercial CD8+ T celll line available. Any recommendation? I want to test cell killing assay.
01 January 2016 6,422 6 View
I want to test the EGFRvIII expression in my cell line. I am wondering if there is any commercial anti-EGFRvIII antibody for flow cytometry? Has anyone have this experience?
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