How to detect transmembrane anti-CD3 antibody?

Hi, I designed a construct encoding extracelluar domain of anti-CD3 antibody and transmembrane domain of CD8. Does anyone have any experience of detect membrane anchored anti-CD3 antibody? what...

07 August 2017 5,863 1 View

How to produce vaccinia virus in large scale for mouse model injectin?

I need to inject 5X10^7pfu vaccinia virus into one mouse. So I have to produce more than 10^9 pfu vaccinia virus. Now I can only produce 10^8 pfu (1ml) virus from 10X150mm tissue plates using...

08 September 2016 922 1 View

Who has A431 cell line?

Who has A431 cell line? I want to use it as EGFR positive control. Can someone borrow me one frozen vial of A431 cell line? We can discuss privately if you have. Thank you

01 February 2016 9,872 0 View

How to test siRNA or shRNA silence efficiency toward PD-1?

I want to test siRNA or shRNA silence efficiency toward PD-1 in whatever cell lines, just to make sure that our siRNA sequence works. Which way is the easiest, WB, FACS or qRTPCR? which cell line...

01 February 2016 3,759 1 View

CD8 T cell line recommendation?

I am wondering if there is commercial CD8+ T celll line available. Any recommendation? I want to test cell killing assay.

31 December 2015 6,366 6 View

Is there any commercial anti-EGFRvIII antibody for flow cytometry recommendation?

I want to test the EGFRvIII expression in my cell line. I am wondering if there is any commercial anti-EGFRvIII antibody for flow cytometry? Has anyone have this experience?

31 December 2015 3,866 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View