Hello!
Recently, CD28 and 41BB Transduced T cells have been cultured in the lab. Our 15 ml cRPMI medium is supplemented with 0.75 IL-15 and 1.5 μl IL-7 for cultivation. The two-week maximal life span in vitro is the issue. That is to say, the cells begin to die after the first week. Any suggestions on how to extend its lifespan?
Many thanks ahead of time..