We found a few CD11b-CD11c+ cells in frozen sections of wounded skeletal muscle. It's day two after wounding and we find no T cells yet, so they most likely won't be T cells. What else could they be? B cells? Can we expect them this early?
Apologies for the bad quality of the picture. I also removed the DAPI so the staining is easier to see. CD11b is red, CD11c green. There are two double stained cells (CD11b+CD11c+, what we were going for) right in the middle.
Hi Daniel, that's great. That's what I was hoping for them to be, but looks very much like I need to shape up my simplistic view of DC. So, there are CD1c+ Cd11b+ and Cd11b- DC infiltrating, or maybe these have been resident before wounding already... more controls then and definitly a lot more reading on DC subsets!
HI,
I am not an expert in skeleton muscle DC phenotypes but if I can do a parallel with the lung, several population of DCs are found.
CD11cCD11b+; inflammatory DCs recruited on the site of the infection.
These can be differenciated from resident CD11b+ lung DCs through their CD14 expression.
Inflammatory CD11c+CD11b+ DCs are CD14+. Lung resident CD11c+CD11b+ are CD14-.
In your case, you may try to stain these cells for their CD14 expression to validate whether there are inflammatory or not.
Hope this help.
This is probably a monocyte-derived DC. Eric Pamer has called these cells Tip-DCs for TNF and iNOS producing DCs. You can also look at Steffen Jung's work on the intestinal DC subsets. These are probably short-lived, transient cells. They may be GM-CSF dependent. GM-CSF can be measured by RT-PCR or ELISA.
They might be CD8a-like DC, attracted by dying cells. In this case, they would be CLEC9A+, XCR1+, CD172a- and CADM1+.
Plasmacytoid DCs, resident CD8+ DCs and migratory CD103+ DCs are CD11b-. You can try specific markers for these subpopulations in your preparations as well
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