How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

Permanent Fluid temperature initialization in Ansys Fluent?

Dear researchers, I am coupling Design Optimization with ANSYS Fluent in Workbench 2020 R2. I can do the analysis. I am patching the initial Temperature values of the zones. However, the patch...

28 January 2021 4,265 2 View

How to couple Moldex 3D with MATLAB for design optimization of an injection mould?

Hello, I would like to use the software Moldex 3D for the design optimization of an injection mould. How can I couple Moldex 3D with a MATLAB code? Any help appreciated, I wish you all a happy...

09 January 2021 569 2 View

How to setup ANSYS Fluent for simulating the filling stage of an injection mould?

Hello, I am using ANSYS Fluent for simulating the filling stage of an injection mould. However, when comparing the results with those of specialized software, such as Moldex 3D, I found the...

07 January 2021 7,992 1 View

Are there any public datasets of consumer complaints about service industries at the European level?

06 January 2021 1,718 2 View

What is the right statistical test?

I have conducted a study in which the participants are divided in two separate groups according to the language of instruction. Also, they are divided in three different groups according to age....

04 January 2021 1,361 17 View

PLS-SEM generalizability issue?

I'm developing a readiness assessment model regarding contractors' preparedness for a specific activity, in order to do so, a survey study was carried out and the data analyzed with PLS-SEM to...

24 November 2020 399 1 View

How to use a .pdb file as structure template to modeling on Chimera 1.14 ?

Hi, I need to use an own file as a template to modeling on Chimera (using Modeller). When I try to blast my sequence only find the option to query structures available on PDB Web Site. Does...

26 October 2020 2,436 3 View

a Ferrari and an Oldsmobile with the same function?

I apologize for the analogy if you are really interested in cars. This is about protein function, but a crucial one because the extraction from membranes of a mistargeted protein is an essential...

15 October 2020 6,147 3 View

What statistical analysis can I use to select individuals from a population of a dioecious species?

I have a population of a dioecious species with significant phenotypic variation and want to select individuals from this population. What statistical methods can I use to perform multi-trait...

11 October 2020 598 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View