Depends on your goals. If you intend to study human parasitic inf.,

(AC16) will be better as it is HUMAN cell line, so the results will be more comparative comparing to mice cell lines such as (H9C2) or (HL-1). Culturing cell line should not be that dilemma as you have a previous experience with culturing techniques in general. You can follow the culturing guidlines easily which come from the manufacture. Notice that any new cell line you use, you will need to adjust your first few runs, so if the price difference between them is big I think going for another cell line is a wise choice. Final issue, try to contact an author who used these cell lines before if you have more doubts regarding their usage.

Good luck

Hello

Yes i agree with Ibrahim Elsharkawi 100%

Good Luck

Does someone know the extension of "H9c2" from H9c2 cell line ?

Hi Salomé, I also work in La Plata (centro de investigaciones cardiovasculares) and there is people here that could assist you if you decide to work with H9C2

Hi

Could anybody be able to provide me with Ac16 cell line, please?

Thanks

Hi Salome Vilchez

If you are familiar with HL-1 cells, then H9c2 cell culture should not be difficult. These cells are quite robust and grow well. You can follow the ATCC protocol to begin with. H9c2 cells can be "differentiated" to a more cardiac phenotype using all-trans retinoic acid. You may take a look at the following papers for the protocol. It is time-consuming but doable. But do verify if your cells undergo differentiation using different methods.

DOI: 10.1074/jbc.274.41.29063

DOI: 10.1371/journal.pone.0129303

DOI: 10.1007/s12012-011-9112-4

AC16 cells are inherently genetically unstable and are resistant to normal transfection agents. Also, they are limited by the usable passage number. Instead, I would recommend human pluripotent stem cell-derived cardiomyocytes. They are expensive but a better model than AC16 cells.

Hope this helps! All the best.

Aneesh

Do H9c2 cardiomyoblasts convert into beating cardiomyocytes?

Soumya Sethi Hi Soumya,

No H9C2 do not beat and they are the myoblast lines.