Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

How to design manholes of a stormwater tunnel?

I am exploring for the design code or manual and standard drawings of access and maintenance manholes of the storm water conveying tunnels. (i.e free flow tunnels not pressurized ones) but all of...

22 December 2020 2,590 4 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

SAS code for a "Split-plot in time" (based on CRD design)?

Dear all, I want to analyze a factorial split-plot in time using SAS. Factorial Experiment using Completely Randomized Design (CRD); Factor A: treatments (a1-a4) Factor B: harvest time,...

28 October 2020 3,515 4 View

Is there a solvent for blending PHB and Acid Ascorbic (via solution casting)?

Dear Researchers I want to add Ascorbic Acid (AA) to PHB in order to improve its biodegradability. AA is soluble in water but insoluble in those that are compatible with PHB (THF, Chloroform,...

07 October 2020 1,136 4 View

Journal vs conference publications, which one would you prefer ?

For sure journal publications have more weight than conference publications but i am asking about your preference. For example i like conferences a lot because i like to meet people and explore...

06 September 2020 7,468 42 View

What is best software for simulation of microfluidic?

I'm working on a microfluidic project. I need a software to simulate all forces include: centrifugal (surely Euler and Coriolis too), and surface tension. Solidworks follow simulation doesn't...

30 August 2020 4,671 9 View

How to simulate hydrogen/air autoignition in CHEMKIN?

Hi everyone, I'm trying to plot hydrogen/air autoignition time vs. inlet temperature. I used a closed homogeneous module in Chemkin. Also, I used Galway hydrogen mechanisms for thermodynamics and...

18 August 2020 4,107 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

03 March 2021 5,360 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View