8 Questions 14 Answers 0 Followers
Questions related from Mostafa Jamshidiha
Hi I'm trying to reconstitute IL-2 from Miltenyi Biotec. The protocol from Miltenyi recommends: " It is recommended to reconstitute lyophilized Human IL-2, premium grade with deionized...
03 December 2018 5,312 2 View
I've been looking into different proliferation assays for CAR T-cells. The protocols mention using 2.5 *10^5 cells for each well. Is that the number of CAR positive cells? If yes, something i've...
24 November 2018 8,020 0 View
Hi I'm trying to study T-cell differentiation using a protocol from a paper. On the panel the authors have used anti-CD27–PE-Cy7 (clone 1A4CD27; Beckman Coulter) and anti-CCR7–FITC (clone...
16 November 2018 3,259 3 View
Hi I'm trying to transduce my CD3/CD28 bead activated purified T-cells. I'm not having much success. I've seen some people use MOI of as low as 1 with VSVG pseudotyped lentiviral vectors (LV)....
09 November 2018 6,844 2 View
Hi I'm trying to activate my T-cells with Dynabeads at a 3:1 ratio. One thing I've noticed is the tendency for the beads to not spread homogenous, whether its in the middle of the well or side of...
02 November 2018 382 4 View
I received a cd3 T cell isolation kit from a Miltenyi distributor with 1 month left on its shelf life. I called them but they said all Miltenyi kits have a shelf life of 6 month. Is this true or a...
20 August 2018 6,096 12 View
Hi I want to use a Endo-free giga prep kit from qiagene. The cells get centrifuged in communal bottles. Apart from autoclaving, do I need to treat them with anything to avoid DNA...
01 January 1970 1,310 1 View
What should be the minimal transduced T cells for cytotoxicity and proliferation assays? Is 30% enough, considering how many positive cells you need to run an assay? thanks
01 January 1970 6,542 2 View
