Can you use SYBR Safe and SRED to quantify RNase activity?

Does anyone have any experience with quantifying RNase activity using SRED (Single Radial Enzyme diffusion)? Papers I have found using it use EtBr, but I was wondering if you could use SYBR Safe...

02 March 2018 1,775 1 View

Is there a speed at which I can centrifuge a sample to pellet eukaryotic cells but keep bacterial cells in the supernatant?

Is there a speed at which I can centrifuge a sample to pellet eukaryotic cells but keep bacterial cells in the supernatant?

02 March 2017 421 4 View

Can I work with staph aureus on the benchtop if I use an open flame?

I have worked with other BSL2 bacteria before (F. nucleatum) and we used an open flame to pick colonies and inoculate cultures. If I am working with staph aureus--i.e., picking colonies to put...

08 January 2017 384 2 View

Can I work with staph aureus on the benchtop if I use an open flame?

I have worked with other BSL2 bacteria before (F. nucleatum) and we used an open flame to pick colonies and inoculate cultures. If I am working with staph aureus--i.e., picking colonies to put...

31 December 2016 1,124 3 View

When performing a semi-dry Western Blot transfer--how important are the sizes of filter papers and membrane, as long as the gel fits on them?

As long as gel has the right thickness of filter above and below it, and the nitrocellulose or PVDF membrane covers my gel, am I just as fine as if everything was cut to the exact same size? If,...

11 December 2016 9,722 1 View

When performing a semi-dry Western Blot transfer--how important are the sizes of filter papers and membrane, as long as the gel fits on them?

As long as gel has the right thickness of filter above and below it, and the nitrocellulose or PVDF membrane covers my gel, am I just as fine as if everything was cut to the exact same size? If,...

05 December 2016 7,992 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How to make RNA extraction process RNase free inexpensively?

We want to extract RNA from virus samples, so we need to make everything RNase free. However, DEPC or RNase cleaning products are out of the question for us at the moment because of their cost. Is...

02 February 2021 2,343 4 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View