Can you use SYBR Safe and SRED to quantify RNase activity?

Does anyone have any experience with quantifying RNase activity using SRED (Single Radial Enzyme diffusion)? Papers I have found using it use EtBr, but I was wondering if you could use SYBR Safe...

02 March 2018 1,775 1 View

Can I work with staph aureus on the benchtop if I use an open flame?

I have worked with other BSL2 bacteria before (F. nucleatum) and we used an open flame to pick colonies and inoculate cultures. If I am working with staph aureus--i.e., picking colonies to put...

08 January 2017 384 2 View

Can I work with staph aureus on the benchtop if I use an open flame?

I have worked with other BSL2 bacteria before (F. nucleatum) and we used an open flame to pick colonies and inoculate cultures. If I am working with staph aureus--i.e., picking colonies to put...

31 December 2016 1,124 3 View

When performing a semi-dry Western Blot transfer--how important are the sizes of filter papers and membrane, as long as the gel fits on them?

As long as gel has the right thickness of filter above and below it, and the nitrocellulose or PVDF membrane covers my gel, am I just as fine as if everything was cut to the exact same size? If,...

11 December 2016 9,722 1 View

When performing a semi-dry Western Blot transfer--how important are the sizes of filter papers and membrane, as long as the gel fits on them?

As long as gel has the right thickness of filter above and below it, and the nitrocellulose or PVDF membrane covers my gel, am I just as fine as if everything was cut to the exact same size? If,...

05 December 2016 7,992 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View