9 Questions 2 Answers 0 Followers
Questions related from Ian Cohn
I am setting up a metagenomics experiment and expect our samples to have low quantities of microbial DNA. We are interested in sequencing blanks as a negative control and to rule out contaminants....
26 April 2018 1,745 4 View
Does anyone have any experience with quantifying RNase activity using SRED (Single Radial Enzyme diffusion)? Papers I have found using it use EtBr, but I was wondering if you could use SYBR Safe...
06 March 2018 9,986 1 View
03 March 2018 1,811 1 View
Is there a speed at which I can centrifuge a sample to pellet eukaryotic cells but keep bacterial cells in the supernatant?
03 March 2017 455 4 View
I would like to perform PCR on crude bacterial lysate. Has anyone successfully done so using lysozyme, and if so what formulation was used?
18 January 2017 6,242 5 View
I have worked with other BSL2 bacteria before (F. nucleatum) and we used an open flame to pick colonies and inoculate cultures. If I am working with staph aureus--i.e., picking colonies to put...
09 January 2017 434 2 View
01 January 2017 1,163 3 View
As long as gel has the right thickness of filter above and below it, and the nitrocellulose or PVDF membrane covers my gel, am I just as fine as if everything was cut to the exact same size? If,...
12 December 2016 9,815 1 View
06 December 2016 8,052 1 View
