Can you use DMSO with Dynabeads His-Tag?

11 April 2023 1 3K Report

I need to purify proteins with HisTag by means of beads fused with NTA. The proteins are in a buffer containing DMSO. After purification no proteins bind the beads, they are in the unbound fraction.

Can DMSO affect the interaction of the HisTag with the NTA-beads?

Engelbert Buxbaum

DMSO can form complexes with transition metals (see, for example, doi:10.1021/ja01508a012, https://en.wikipedia.org/wiki/Transition_metal_sulfoxide_complex). In addition, the protein reacts to DMSO in a concentration dependent manner (unfolding, tighter folding...).

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