Claudia D'Ercole

4 Questions 2 Answers 0 Followers

Questions related from Claudia D'Ercole

Which imidazole with a lower signal at 280nm can I use during IMAC purification?

Hi, I purify protein by IMAC purification by increasing imidazole concentration. However, in the chromatograph sometimes it is difficult to see a clear peak from A280 of protein beacuse of the...

24 July 2023 5,908 3 View

Can you use DMSO with Dynabeads His-Tag?

I need to purify proteins with HisTag by means of beads fused with NTA. The proteins are in a buffer containing DMSO. After purification no proteins bind the beads, they are in the unbound...

11 April 2023 2,784 1 View

Best substrate of Alkaline Phosphatase?

Hi everyone! I'm doing an ELISA test with Streptavidin conjugated with Alkaline Phosphatase. What is the best substrate? I’d rather not use fluorescent substrates. Many thanks!

27 March 2023 4,943 2 View

What is the shape of TG1 E.Coli cells?

I'm working with E.Coli TG1 strain. I think I have a contamination, maybe they are mold or rod bacteria. I checked under the microscope and I can see big rods. TG1 are E.Coli so I should see...

11 January 2023 1,295 3 View