Sheikh Gulfam

6 Questions 14 Answers 0 Followers

Questions related from Sheikh Gulfam

How to collect references of a manuscript automatically by ONLINE mode ?

I am unable to install properly the EndNote, Mendeley or Zotero. I have a software problem in my PC. So, i need an online tool for collecting the references.

01 November 2017 3,572 4 View

Can you suggest me why we remove stop codon when protein sequence is to be analysed and classified in Interpro?

Hello dear friends Can anyone suggest me, please , why we remove stop codon when protein sequence is to be analyzed and classified in Interpro? When i search the sequence with stop codon i m...

27 April 2017 4,174 2 View

What is the procedure for making phylogenetic tree from short contig sequences with protein sequences ?

I am trying to make phylogenetic trees of ABC transporter protein sequences(actually translated contigs - transcriptome data) of Coleus with ABC transporter protein sequences of Arabidopsis...

24 March 2017 1,182 1 View

What is the recommended concentration range of elicitors/ hormones like 2,4-D, ABA, Salicylic acid and MeJA to create stress conditions for plants ?

Hello Friends, Can u tell me the recommended concentration range of these hormones for stress treatment to plants like Coleus forskohlii

07 February 2017 8,945 10 View

What are the critical factors which hinder/inhibit the cDNA synthesis ?

Dear friends , I isolated the total RNA from Coleus stem and the RNA looked good on gel (below). I made the cDNA from the RNA but when i checked PCR with Actin(250bp) and gene specific primer, it...

14 December 2016 1,187 1 View

Why my PCR product sometimes shows smear and sometimes bigger size than should be ?

My problem is related with ITS primer .Previously i had no problem but from fews days bands on 1% agarose gel sometimes show smears, sometimes multiple bands and sometimes increase in amplicon...

07 November 2016 9,414 6 View