Sheikh Gulfam

5 Questions 12 Answers 0 Followers

Questions related from Sheikh Gulfam

Can you suggest me why we remove stop codon when protein sequence is to be analysed and classified in Interpro?

Hello dear friends Can anyone suggest me, please , why we remove stop codon when protein sequence is to be analyzed and classified in Interpro? When i search the sequence with stop codon i m...

27 April 2017 4,169 2 View

What is the procedure for making phylogenetic tree from short contig sequences with protein sequences ?

I am trying to make phylogenetic trees of ABC transporter protein sequences(actually translated contigs - transcriptome data) of Coleus with ABC transporter protein sequences of Arabidopsis...

24 March 2017 1,178 1 View

What is the recommended concentration range of elicitors/ hormones like 2,4-D, ABA, Salicylic acid and MeJA to create stress conditions for plants ?

Hello Friends, Can u tell me the recommended concentration range of these hormones for stress treatment to plants like Coleus forskohlii

07 February 2017 8,939 10 View

What are the critical factors which hinder/inhibit the cDNA synthesis ?

Dear friends , I isolated the total RNA from Coleus stem and the RNA looked good on gel (below). I made the cDNA from the RNA but when i checked PCR with Actin(250bp) and gene specific primer, it...

14 December 2016 1,182 1 View

Why my PCR product sometimes shows smear and sometimes bigger size than should be ?

My problem is related with ITS primer .Previously i had no problem but from fews days bands on 1% agarose gel sometimes show smears, sometimes multiple bands and sometimes increase in amplicon...

07 November 2016 9,409 6 View