What is angular velocity of projection of uniform circular motion?

12 November 2020 481 5 View

Ways to establish exchange bias in ferromagnet/ antiferromagnet heterostructure?

When ferromagnet (FM) and antiferromagnet (AFM) are coupled, exchange bias can be generated at the interface of FM and AFM which acts as a bias field in the hysteresis loop. I am particularly...

24 October 2020 8,580 1 View

How to wash the alumina or AlN substrate sidewall after laser cutting?

With ultrafast laser we normally get a smooth and white sidewall and edges of laser cutting, but for some thick ceramics substrates we have to cut with fiber or CO2 lasers, and there is burs and...

07 July 2020 977 3 View

Can we use TargetScan to predict targets of a novel miRNA sequence?

Hi all,we obtained some mutated miRNA sequences and planned to predict their target via TargetScan,but the website of TargetScan only recognize rna names,which means that miRNA not included in...

27 May 2018 4,533 2 View

How to keep flow-sorted live CD45+ cells from mouse intestines alive for longer?

We have been trying to flow sort live CD45+ cells from mouse intestines. One major issue is that these live cells continue dying after sorting. This is very critical for our following experiments...

02 September 2017 4,347 3 View

Simulate Rayleigh channel using Matlab

I use following code to simulate a QPSK signal through 3-path Rayleigh channel, signal_r=2*(rand([Ls,1])>0.5)-1; signal_i=2*(rand([Ls,1])>0.5)-1;QPSK=signal_r+1i*signal_i; Es=((QPSK)'...

27 June 2017 6,388 9 View

Does anybody publish work in stress and inflammation biomarkers?

Does anybody publish work in stress and inflammation biomarkers (allostatic load measures, hsCRP, IL-6, MCP-1)? I am looking for a biological collaborator for my R grant in 'care for wife caregivers.'

31 October 2016 6,385 5 View

Culturable microbiome NGS?

My lab has done 16S and whole genome shotgun on mouse gut/fecal microbiome (wild-type vs knockout). Recently we found that the culturable part of these knockout mouse fecal samples are actually...

30 June 2016 8,839 4 View

Do monocytes can be stained with Cy7 NHS ester?

Actually, Cy7 NHS ester can be used in staining peptides. I want to stain the peptides that can bind to monocytes. Hence, I used Cy7 NHS ester for labeling the peptides and DAPI for labeling...

03 January 2016 2,071 1 View

Can anyone suggest the dosage of irradiation for DC?

I am trying to co-culture the mouse DC with lymphocytes, and I wish to irradiate the DC prior the co-culture, what would be the optimal dosage of irradiation?

07 April 2014 9,812 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View