We are developing standard methods to quantify microorganisms in soft drinks. The procedure we use is the filtration method. However, for some soft drinks, the presence of stabilizers and thickeners make it hard for filtration and the presence of carbonation make it hard for pipeting. Is there an easy standard method for those type of drinks? Thanks
Carbonation may affect your pipetting. Place in a sterile (or laminar flow among burners) with a glass rod could eliminate all gas. You could use the methodology of total count (total aerobic mesophilic, it indicates the degree of contamination) using standard methods agar. To pathogens check your regulations to see that you requested, but you could determine total coliforms by the most probable number technique.
Get rid of the carbondioxide totally and then for the total count you can do the TPC thing and for the pathogens you can do the MPN 5 tube or 10 tube method. Finally by doing the completed test for E. coli (Indol test or grow it on EMB agar) you can finally say the presence of E. coli can indicate contamination by faeces, hide etc and thus other pathogens may also present.
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