RNA extraction from bacteria culture ?

Hi everyone, I am currently working on RNA extraction from bacterial (gram-neg) cultures for whole-transcritpome sequencing. I use a protocol as follow: Bacterial cell pellets are resuspended in...

11 November 2020 4,842 4 View

How do I determine the correct ratio to peak value for the Recession baseflow in HEC-HMS?

I am wondering if there is a way to estimate the ratio to peak value without calibration? What would be the most suitable value if I don't have a measured hydrograph to compare? Thanks for any...

27 July 2020 2,242 1 View

How do I estimate initial baseflow for each subbasin (HEC-HMS) ?

I have one hydrograph close to the watershed outlet from which I can extract a baseflow value. But what values would I assign to all the other subbasins? The HEC-HMS user manual says the...

21 July 2020 5,431 3 View

Why is my basin model file exported from HEC-GeoHMS empty?

I realise this is a very specific question but maybe there is someone out there who experienced this issue before and could solve it somehow. I've created a model in ArcGIS with the help of...

13 July 2020 8,499 3 View

Regression with one dummy-coded IV not significant?

Hi together, I am looking for an answer to an SPSS related question, using only ONE dummy coded IV with two groups. Other than that my model only includes moderators and control variables. After...

23 September 2019 2,522 5 View

Can you help me with the steps to make a SASfit fitting?

I have to fit long cylinders structures using the SASfit package. I would like to know which are the best reasonings (steps) to make a good fit? What do I have to consider when I'm fitting the...

29 May 2019 8,520 2 View

Database program for animal experiments?

Hi! I'm involved in a animal experiment that will generate a lot of data over several years. There will be e.g. blood parameters that are investigated over several weeks, cell culture work from...

22 October 2017 7,535 4 View

Database program for animal experiments?

Hi! I'm involved in a animal experiment that will generate a lot of data over several years. There will be e.g. blood parameters that are investigated over several weeks, cell culture work from...

09 October 2017 8,053 5 View

260/280 Ratio increased after DNAse treatment?

Total RNA was extracted from FFPE blocks and concentrations were measured using nanodrop. 260/280 looked normal (1.89-2.06). I treated these samples with DNAseI from Invitrogen and my ratios...

19 September 2017 3,251 2 View

[Germany] funding opportunities for own position of young postdocs/Finanzierung eigene Stelle PostDoc?

Dear all, my funding source could not be prolonged for the time of my maternity leave. In the meantime i applied to some stipends (mainly addressing women restarting their career) but did not get...

09 October 2016 5,251 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View