Dear fellow bone researchers,
i wanted to perform some cell culture experiments using intermittent PTH treatment on human MSCs. I have tried many different conditions but haven't succeded in increasing mineralization (or ALP). Can you share a successful protocol/publication with me please?
So far i have tried:
- adjusting the dose (0.1nM - 100nM)
- adjusting the treatment duration within each cycle (1h-6h treatment per 48h cycle)
- adjusting the PTH treatment period (during the complete differentiation period vs different time frames (5d each either early, intermediate or late during differentiation)
Only thing that works is that continous treatment completely abolishes mineralization. But all iPTH conditions always result in less mineralization compared to the osteogenic medium alone.
What else can i adapt to finally recapulate the anabolic effect of PTH?
Best,
Juliane
Hi Juliane,
That's really hard to doe reliably. FSK works better for us, esp if low dose BMP added. See wildtype cells in Fig 2g-j of PMID:23963683
Best regards,
Ed
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