Try  Alkaline SDS. 1% SDS and 0.2N NaOH. Prepare it fresh each time for DNA isolation. I hope it works for your assay. If you can, please share your protocol so that I can suggest more modifications.

Hi Fedor,

I have experiences only with Sf9 insect cell line, where I use for lysis PBS with 1 % Triton. For entire insect I am not sure, but  I would use SDS method described here: http://dx.doi.org/10.1371/journal.pone.0011963. I never done this, my recommendation is based on our work with soil DNA isolation.

Best regards,

                Marian. 

Hi Fedor,

for crustaceans, beetles and dipterans we use successfully and for many years the Queen’s lysis buffer - 200ul with 5 ul of proteinase K (20 mg ml-1) per sample. The buffer is described by Seutin G, White BN, Boag PT (1991) Preservation of avian blood and tissue samples for DNA analysis. Can J Zool 69:82–90.

If you want we may sent you the recipe.

Best,

Michal

Hi Fedor, see attachment for the protocol that is our cheap and golden standard for many projects. 

https://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-016-0723-z

Hi Florian, seems to be a nice option. Do you have any idea about the recovery ration and stability of DNA extracted with this method? Can it be stored for a long time?

Best,

Michal

Dears,

thanks a lot for you assistance. It was very useful for me.

Fedor

Hi Michal, it can be stored for ages if stored in normal elution buffers with adjusted pH (8.0 typically). Very good experiences and using the mentioned Sunnucks & Hayes salt-precipitation protocol you (Fedor) can omit the Phenol-Chloroform which is great in terms of yield but suboptimal in terms of general handling.