We are new to metabarcoding from sediment samples, and so would greatly appreciate suggestions for protocol how to process sediment samples to get DNA. Our samples come from freshwater habitats. Thanks
Through crystallized fossils
Dears, I am working with beetles and used to extract DNA mostly with kits. Now we extract with phenol-chloroform, but I would need to prepare a Lysis buffer. I am more entomologist than chemist,...
10 November 2016 3,011 7 View
Even I have tried several kits and protocols, I almost always fail to extract DNA from the museal specimens (ca 10-20 years old) of beetles. I would need to amplify commonly used fragments like...
31 December 2014 5,360 6 View
I would very appreciate any suggestions about primers for cytochrome b, that work well on Trichoptera samples. We used some primers from Simon et al 1994, but with very low success. The template...
05 June 2014 2,017 4 View
I am working on taxonomy and phylogeny of the riffle beetles (Coleoptera: Elmidae), using molecular characters. Due to that I try to get as many elmid samples from all around the World, as...
01 February 2013 9,247 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...
23 July 2024 1,672 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View