Swapnil Muley

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Questions related from Swapnil Muley

Which is the most suitable method for amplifying the titre of phages displaying scFV?

I have cloned scFV against various target proteins by infecting XL-1B cells containing phagemid pSEX81 with M13 hyperphage (Progen protocol http://www.progen.de/hyperphage.html). The titre...

11 November 2016 2,623 4 View

Can anyone help in standardizing ELISA panning in phage display with M13 hyperphage?

I am working on recombinant antibody technology, where phages are used for displaying antibody fragment on the surface. I am able to clone the antibody gene successfully in phagemid vector pSEX81,...

11 November 2016 7,352 8 View

How to decide the molar ratio for vector:insert for ligation reaction?

I am using a vector of size 4.8 kb having conc. of 100 ng and my insert size is around 1kb. Online plugins are available for calculating how much insert should be used, but I want to know which...

11 November 2016 3,158 6 View