5 Questions 11 Answers 0 Followers
Questions related from Fedor Čiampor Jr
We are new to metabarcoding from sediment samples, and so would greatly appreciate suggestions for protocol how to process sediment samples to get DNA. Our samples come from freshwater habitats....
07 July 2019 5,239 1 View
Dears, I am working with beetles and used to extract DNA mostly with kits. Now we extract with phenol-chloroform, but I would need to prepare a Lysis buffer. I am more entomologist than chemist,...
11 November 2016 2,986 7 View
Even I have tried several kits and protocols, I almost always fail to extract DNA from the museal specimens (ca 10-20 years old) of beetles. I would need to amplify commonly used fragments like...
01 January 2015 5,326 6 View
I would very appreciate any suggestions about primers for cytochrome b, that work well on Trichoptera samples. We used some primers from Simon et al 1994, but with very low success. The template...
06 June 2014 1,994 4 View
I am working on taxonomy and phylogeny of the riffle beetles (Coleoptera: Elmidae), using molecular characters. Due to that I try to get as many elmid samples from all around the World, as...
02 February 2013 9,221 2 View
