We have detected novel miRNAs in our sample where cut off score falls between 0.4 to 2.4.
Check out http://www.nature.com/natureevents/science/events/47533-microRNA_Analysis_Using_Next_Generation_Sequencing
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04 May 2016 2,117 1 View
To study functionality of miRNAs We are overexpressing miRNA-122 in the cells using lipofectamine. We want to cotransfect mimics as well as inhibitors. So how shall I go about it? Kindly share...
31 December 2015 7,848 3 View
How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.
31 December 2014 9,900 9 View
We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.
31 December 2013 4,548 3 View
Differentiation of MSCs.
09 October 2013 7,984 18 View
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05 June 2013 9,786 4 View
Why 95% reseachers use Low Glucose DMEM with only 2mM of glutamate (instead of 4mM what we normally use) to culture human umbilical cord derived mesenchymal stem cells? is there any specific...
31 December 2012 4,653 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
it is required to differentiate deep learning and ANN
31 July 2024 9,442 4 View
I am seeking a PhD position in the area of Deep Learning, specifically in generative AI. Cn anyone of you help me finding a suitable supervisor open for a PhD candidate.
28 July 2024 1,849 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View