We have detected novel miRNAs in our sample where cut off score falls between 0.4 to 2.4.
Check out http://www.nature.com/natureevents/science/events/47533-microRNA_Analysis_Using_Next_Generation_Sequencing
-
04 May 2016 2,035 1 View
To study functionality of miRNAs We are overexpressing miRNA-122 in the cells using lipofectamine. We want to cotransfect mimics as well as inhibitors. So how shall I go about it? Kindly share...
31 December 2015 7,730 3 View
How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.
31 December 2014 9,806 9 View
We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.
31 December 2013 4,461 3 View
Differentiation of MSCs.
09 October 2013 7,877 18 View
...
05 June 2013 9,709 4 View
Why 95% reseachers use Low Glucose DMEM with only 2mM of glutamate (instead of 4mM what we normally use) to culture human umbilical cord derived mesenchymal stem cells? is there any specific...
31 December 2012 4,561 2 View
What Characteristics makes CNN work better?
03 March 2021 1,458 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
L1 and L2 regularization
28 February 2021 4,187 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...
24 February 2021 8,856 3 View