8 Questions 23 Answers 0 Followers
Questions related from Akshata Raut
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05 May 2016 2,077 1 View
We have detected novel miRNAs in our sample where cut off score falls between 0.4 to 2.4.
05 May 2016 9,475 1 View
To study functionality of miRNAs We are overexpressing miRNA-122 in the cells using lipofectamine. We want to cotransfect mimics as well as inhibitors. So how shall I go about it? Kindly share...
01 January 2016 7,811 3 View
How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.
01 January 2015 9,856 9 View
We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.
01 January 2014 4,511 3 View
Differentiation of MSCs.
10 October 2013 7,946 18 View
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06 June 2013 9,755 4 View
Why 95% reseachers use Low Glucose DMEM with only 2mM of glutamate (instead of 4mM what we normally use) to culture human umbilical cord derived mesenchymal stem cells? is there any specific...
01 January 2013 4,618 2 View