Akshata Raut

8 Questions 23 Answers 0 Followers

Questions related from Akshata Raut

How to perform target prediction analysis for novel miRNAs?

05 May 2016 2,078 1 View

Can you please help me to understand miRdeep cut off score for novel miRNAs?

We have detected novel miRNAs in our sample where cut off score falls between 0.4 to 2.4.

05 May 2016 9,476 1 View

Can I co-transfect adherent cells with miRNA mimics and miRNA inhibitors ?

To study functionality of miRNAs We are overexpressing miRNA-122 in the cells using lipofectamine. We want to cotransfect mimics as well as inhibitors. So how shall I go about it? Kindly share...

01 January 2016 7,813 3 View

How to interpret Real time PCR data?

How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.

01 January 2015 9,856 9 View

Can you suggest a list of microRNAs which are specific only to mesenchymal stem cells, irrespective of its origin?

We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.

01 January 2014 4,512 3 View

Why is chondrogenic differentiation of MSCs done in pellet culture?

Differentiation of MSCs.

10 October 2013 7,946 18 View

What is best way to carry out microRNA profiling? Also what is the best kit to extract microRNA from primary cell lines?

...

06 June 2013 9,756 4 View

Media for Umbilical cord Derived Mesenchymal stem cells

Why 95% reseachers use Low Glucose DMEM with only 2mM of glutamate (instead of 4mM what we normally use) to culture human umbilical cord derived mesenchymal stem cells? is there any specific...

01 January 2013 4,618 2 View