Sally Irwin

10 Questions 12 Answers 0 Followers

Questions related from Sally Irwin

Possible solutions OD595 Absorbance reading ?

We are trying to pick up an effect on lysozyme by measuring the absorbance of florescent labeled M. luteus cells at OD600. We are using this as a substrate because we are comparing the results...

03 December 2024 6,902 4 View

Has anyone used the new E1 Clip tip digital pipettes?

We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...

02 July 2024 2,099 0 View

Why would Vmax and Km not change when inhibitor shows clear reduction in activity?

We have been working with an inhibitor that clearly and consistently reduces an enzymes product by about 25-30%. However in trying to elucidate the mechanism using Michaelis-Menten and...

15 April 2024 4,972 5 View

Can you help with question about MIchaelis Menten graphs for Lysozyme activity?

We have been studying the effects of some inhibitors on lysozyme activity. When we look at a graph of product (fluorescence) over time we see a clear inhibition of the enzyme activity that...

06 November 2023 9,869 1 View

Has anyone ever used a speed vac to dry bacterial pellets?

This is not for DNA prep, I want to keep the cells intact.

26 September 2023 6,064 2 View

Are the Micrococcus lysodeikticus cells in the enz check lysozyme assay intact?

We have been using the Enz Check lysozyme assay and are assuming the cells are not intact but in pieces. However over time and especially with vortexing or pipetting to mix substrate, I would...

03 October 2021 3,537 1 View

What company or university lab do you recommend to sequence specific bacterial species from a human microbiome sample?

We are looking for a lab to send human microbiome samples to that can quantify specific bacterial types from . We need a company you have found to be reliable, cost effective and reputable.

08 October 2019 374 4 View

Has anyone figured out a way to avoid condensation on 96 well plates in plate reader?

We are trying to assay bacteria growing at 37 C in plate reader over time and are having condensation problems. Any ideas?

26 April 2017 1,252 5 View

Has anyone used the 6x6 drop plate method for bacterial enumeration? If so how do you do it so that the 10 ul dots don't spread into each other?

I am trying to do bacterial counts on MRS plates using the drop plate method which uses a dilution series and 10 ul drops on the plate.

28 August 2016 7,255 7 View

How do I measure the growth curves on some Lactobacillus species?

I want to develop growth curves for three different Lactobacillus species. They all seem to grow fairly well on MRS media at 35 C and 5% CO2 but not that well in MRS broth under these same...

30 March 2015 6,853 3 View