Bacteriocin production is being optimized. However, pH, agitation rate, temperature and time all proceeded under the same conditions. In addition, the condition for growing the bacteria was that the colony was inoculated into the tsb broth, incubated overnight, then inoculated 1% and cultured for 24 hours. However, when the experiment is conducted using the well-diffusion method, 19mm is sometimes measured and sometimes 16mm is measured. The results are not identical, so I'm looking for a problem and can you give me your feedback?
Hi,
How numbers of 1%? I think, the bacteria number is different .
In order to optimize bacteriocin production for industrial applications a study evaluated the effect of supplementation of MRS broth with glucose, Tween 80, sodium citrate, potassium chloride and cysteine and effect of initial pH. Factors such as temperature, pH and media composition can influence the level of bacteriocin produced.Response Surface Methodology analysis indicated that the highest bacteriocin occurred in the MRS broth supplementation with 5.5 g /L glucose and 1.05% Tween 20 at an initial pH OF 6.28 and incubation temperature of 25 oC. For details consult www.scielo.br --scielo
Niamah, thank you for your reply. i inoculated 1% of overnight culture. The CFU of the overnight culture is about 2~5 X 10^9 CFU/ml. Can a difference of 2 to times (CFU/ml) make a difference in results??
Antai, tank you for your reply. we plan to do optimization through RSM technique. However, the current problem can not be reproduced even if we proceed to the conditions that we have already established. So it seems to have to find a factor for it.
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