Hello everyone I am looking for recommendations on a good protocol for fractionation and immunostaining of human fibroblasts.
How can I add additional published work?
03 June 2024 4,045 2 View
Greetings everyone, I am doing a research project on the effect of the glass ceiling on the work performance of women engineers in the construction sector. Do you know of any questionnaire that...
01 June 2024 7,226 0 View
Fractionation 1. Scrape cells and collect by centrifugation (500 × g, 5 min, 4 °C) 2. Resuspend cell pellets in lysis buffer and incubate on ice for 10 minutes 3. Centrifuge at 500 x g for 5 min...
25 March 2024 6,347 3 View
I am writing a master's paper looking at the connection between learning and physical movement for students with disabilities. Have you run across any recent studies or information?
12 March 2024 835 0 View
I want to dry down a large quantity of liposomes and aliquot and freeze. I want to be able to play around with the inside conditions, changing buffers, dyes, etc. I can make a 2X buffer to hydrate...
11 March 2024 7,157 0 View
Pretendo investigar cuál sería el método que más se adapta a la investigación, todo ello desde una perspectiva cuantitativa y cualitativa. Teniendo en cuenta los estereotipos de género y las...
11 March 2024 7,376 0 View
Use of metronidazole in leg ulcers
01 March 2024 5,775 2 View
non pharmacological treatment for infected leg ulcers
01 March 2024 8,671 2 View
I used: .01M Phenylalanine. .1 M Ascorbic acid
20 February 2024 6,790 0 View
I'm trying to scan the surface of E. faecalis biofilms on glass slides however when using AFM in liquid mode, I get nothing. My theory is that E. faecalis produce weak biofilms that lift off as...
20 February 2024 4,959 0 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Please explain the protocol in detail along with the reagents to be used
03 July 2024 876 1 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,866 2 View
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View
Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable
15 June 2024 3,010 2 View
Hi everyone, I am working with an adhesive fibroblast cell line in roller flasks (Cell Master Bottles). When culturing the cells with FBS that is heated with FBS (around 60 degrees C), the cells...
02 June 2024 7,409 1 View
I started a cell culture with a mix of suspended and adherent cells (presumably fibroblasts) on May 10th. After a week, I treated them for adherent cells only. On May 24th, most of the adherent...
02 June 2024 4,903 5 View
Recently I've been doing some indirect co-culture experiments, in a nutshell, treating cancer cells with CAF's conditioned medium. So how do you do the collection of conditioned media and the...
01 June 2024 9,484 2 View