How to write multiple hypotheses?

This is what I have for my hypotheses at the moment - is this an okay to write it or how should I format it (this makes sense to me but I haven't seen hypotheses written like this before)? Any...

15 November 2020 994 2 View

Lipoic acid with a terminal amine causing gelation?

I am wondering if anyone has made Lipoic acid with a terminal amine and has seen gelation? Is there anyway around this or a way to get it back into solution?

29 October 2020 2,839 1 View

Should I include irrelevant parts of my methodology?

I am writing up a project in which I used motion capture as well as heart rate monitoring and questionnaires, but I will be analyzing the motion capture data separately to this project write up -...

23 October 2020 6,796 5 View

Is my research title too complex?

Research title: The effectiveness of gamified virtual reality for increasing adherence to exercise, affecting perceived exertion and improving mental and physical health: A preliminary study...

18 October 2020 4,505 10 View

Can you do multivariate multiple linear regression in AMOS?

Hello there, I have been struggling to wrap my head around which statistical analysis to use for my research question: I have 17-20 potential outcome variables (subtests on a cognitive battery,...

10 September 2019 9,953 3 View

Flood Prediction: Where should my starting point be?

Hi, I'm currently studying computer science and I am working on an Internet of Things project involving the use of rainfall sensors in different locations. I'm currently researching if these...

28 January 2019 864 5 View

What are your suggestions to close up a vector after removing a fragment by restriction digest?

Dear community, I am working with a HUGE protien, in a plasmid we received from collaborators. It is 20,880 bp in size, and the gene of interest is aprox. 15,600 bp. Thankfully the received...

20 August 2018 9,664 4 View

When dissolving expanded polystyrene (EPS) in acetone what fumes are produced? And is there a way of measuring this?

I am researching and developing alternate methods of recycling EPS and am getting interesting results via dissolution in acetone. However I want to make an evaluation of the potentially hazardous...

15 January 2018 478 2 View

Can anyone give advice for doing mixed ANOVA with an imputed data set?

I have successfully imputed missing data in my data set but I am struggling to follow VanGinkel & Kroonenberg's instructions for what to do next. Does anyone know is there an easy way to do...

12 March 2017 4,817 3 View

What sample size would I ideally need for an in vitro tissue permeability model study?

I am starting a project using Ussing chambers to measure tissue TEER and permeability for injured vs noninjured tissue. I was wondering what the minimum sample size is for such studies?

17 September 2016 8,670 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View