Hi! I am measuring changes in glucose uptake in primary adult cardiomyocytes after addition of fatty acids. I am using a 1:100 dilution of CD lipids. Glucose uptake is measured by fluorescent 2-DG. Currently with an n=3 mice, 5 technical replicates per mouse, I observe that glucose uptake increases with addition of lipids, but according to the literature, it seems like the opposite should happen. I cannot find a study that directly looked at fatty acid induced glucose uptake, only ones that found fatty acid oxidation inhibits insulin mediated glucose uptake, or high glucose uptake promotes lipogenesis. Could someone please share their expertise? Any help is appreciated. Thank you.
Boden, G., Chen, X., Ruiz, J., White, J. V., & Rossetti, L. (1994). Mechanisms of fatty acid-induced inhibition of glucose uptake. The Journal of clinical investigation, 93(6), 2438–2446. https://doi.org/10.1172/JCI117252
Effects of Free Fatty Acids on Glucose Uptake and Utilization in Healthy Women | Diabetes | American Diabetes Association (diabetesjournals.org)
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