Can Wright-Giemsa stain be used on suspended cells?

27 April 2023 1 7K Report

I am looking to stain blood cells in suspension in order to use in a flow cytometer. Is there protocol for staining suspended cells in culture media or PBS? Thank you!

Antonio La Gioia

I think not. All staining procedures based on the Papanicolau method require a preventive fixation with methanol that "in vivo" would destroy the cells.

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