I am working on drug target, I have to do spectrophotometry enzyme assay and enzyme kinetics of this protein in 260nm. So I don't have uv transparent plate. So, can we use clear bottom Corning 96 well plate for this assay in this range (far uv) 260nm of wavelength.
Hello Akhilesh kumar Gupta
No, you cannot use clear bottom Corning 96 well plate for the assay in the far UV range.
Clear bottom Corning 96 well plate is made up of polystyrene. Conventional polystyrene or polypropylene microplates, while quite adequate for absorption determinations in the visible light portion of the spectrum, are unsuitable for determinations in the UV range. Polystyrene absorbs UV especially in the short-wavelength range (< 320 nm). So, because of their relatively high background absorbance, these plates are unacceptable for use in determinations that require measurements in the ultraviolet portion of the spectrum.
The disposable “UV transparent” microplates demonstrate much better absorbance characteristics in the UV wavelengths than conventional plastic microplates. Therefore, use of “UV transparent” microplates is recommended for the spectrophotometric enzyme assay and enzyme kinetics at 260nm.
For more information, you may want to refer to the link below.
https://www.agilent.com/cs/library/technicaloverviews/public/PowerWave200-absorbance-measurement-5594-3409EN-agilent.pdf
Best,
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