No, whereas cyanide salts are among the most rapidly acting of all known poisons. Cyanide is a potent inhibitor of respiration, acting on mitochondrial cytochrome oxidase and hence blocking electron transport. This results in decreased oxidative metabolism and oxygen utilization and is not reversible secondary to its high affinity of cyanide for heme group containing proteins including cytochrome C. I would suspect you could generate a metabolic dose response curve using cyanide however oxygen depravation using a dose response curve (O2 concentration in chamber) is a different mechanism of oxygen depravation than with cyanide. In addition, you can evaluate cell recovery post O2 depravation by hypoxia which can not be done with cyanide (non reversible).
No, whereas cyanide salts are among the most rapidly acting of all known poisons. Cyanide is a potent inhibitor of respiration, acting on mitochondrial cytochrome oxidase and hence blocking electron transport. This results in decreased oxidative metabolism and oxygen utilization and is not reversible secondary to its high affinity of cyanide for heme group containing proteins including cytochrome C. I would suspect you could generate a metabolic dose response curve using cyanide however oxygen depravation using a dose response curve (O2 concentration in chamber) is a different mechanism of oxygen depravation than with cyanide. In addition, you can evaluate cell recovery post O2 depravation by hypoxia which can not be done with cyanide (non reversible).
I agree with Frank, CN will not give the same result as hypoxia. You will have to use a chamber where you can tightly regulate O2 levels.
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