Can I use Sybr green detection method to quantify, miRNA cDNA prepared using the Taqman advanced miRNA cDNA kit. If yes, can i use Qiagen's-miRCURY LNA miRNA PCR Assays (Real time-qPCR related primers) with cDNA synthesized from taqman miRNA cDNA kit. Would there be bias between the primers designed between taqman and qiagen as I presume the tags may be different during the reverse transcription process for each kit. Thanking you in advance.