Which ethical metrics is expected from an academic who is the owner or the creator of the measuring instrument?

Which ethical metrics is expected from an academic who is the owner or the creator of the instrument? The measurement tool will be applied to adults as an online form and a snowball sample will...

01 February 2021 9,188 3 View

Hi my name is syahida. may i know the accurate concentration of lpg gas?? is it 250 ppm consider as dangerous gas in household?

hi my name is syahida. may i know the accurate concentration of lpg gas?? is it 250 ppm consider as dangerous gas in household? can you guide me to do the project of lpg gas detector system...

23 January 2021 9,425 4 View

What the reliability method of filling missing time series data?

Hi Researchers, How to measure the reliability of the method for filling missing time series streamflow data such as ARIMA model and interpolation?

12 January 2021 2,725 7 View

How to standardize and set parameters in the Puller sutter P-97 to get accepted pipette shape and resistance for whole cell recordings on DRG?

We are working on a P-97 puller and we are using it to do whole cell recording in DRG. We have 2 types of pipettes (borosilicate glass ID: 1.1 OD: 1.5 and ID: 0.86 OD: 1.5 ) as it showed in the...

04 January 2021 2,112 6 View

How do I write in a more professional scientific way?

I am an undergraduate in university. I am new to scientific research and would like to know how to make my paper more professional. Thanks in advance.

04 January 2021 264 12 View

What is the difference between Introduction, Background, Literature Review and Conclusion for writing a Thesis Report?

I've recently started writing a thesis report based on my research paper. And I'm very confused about writing the chapter of the literature review because we already discusse everything in the...

03 January 2021 6,028 8 View

In the case of SEM (scanning electron microscopy) of Fasciola gigantica, some white spots/substances were found but what are the causes for these?

In the case of SEM (scanning electron microscopy) of Fasciola gigantica, some white spots/substances were found but what are the causes for these?

03 January 2021 8,525 3 View

Does "response to item ratio" has any effect on "inter-item correlation"?

Suppose a survey was conducted with a lower sample size (less than 100) for factor analysis of the items (less than 20). Now would a larger sample size increase the inter-item correlation? What...

27 December 2020 1,722 3 View

What is the most perfect way to convert Latex to Word?

I am looking forward to finding the best way to transfer Latex files to word format. I found some technical ways but they do not work perfectly for some sections such as tables and formulas....

06 December 2020 6,530 5 View

How can one integrate gender into designing any architectural space? are there any examples of it in the architectural practice?

I am doing my undergrad thesis on gender integration in architecture and could use a few views from people with different perspectives.

03 December 2020 8,378 11 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View