Can we use C18 column instead of C20 column for ethanol estimation in HPLC?

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Yeghig Armoudjian

do a GC and rest your mind :P but yes you can use C18 but you might consider the column length or other organic percentage in your mobile phase .

Abdur Mirajkar

Yeghing is right .You should do GC .It fast and accurate.

Bruce Neagle

HPLC would not be my 1st choose! Ethanol is invisible in the UV so a PDA/DAD will not work. In addition, it is an organic solvent and are not retained by C18 columns.

You would have to use a Waters Sugarpak specialty column (or equivalent, $$$) and depending on your sample matrix and expected concentration RI or ELSD detector.