Can we use bovine PPD on human PBMCs?

20 August 2021 1 6K Report

We are using PPD as a stimulant for proliferation assays (ELISpot and Thymidine) on human PBMCs and are having trouble finding more PPD. Has anyone used bovine PPD on human PBMCs in assay?

Martin Waterfall

Hello Vanessa, There are several publications by Lesslie, I.W. comparing tuberculin purified protein derivative sources with immunological reactivity in tb surveillance that you would find useful.

Comparison of the specificity of human and bovine tuberculin PPF for testing cattle. 3. National trial in Great Britain - https://europepmc.org/article/MED/47752.

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