Vanessa Barriga

5 Questions 1 Answers 0 Followers

Questions related from Vanessa Barriga

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

17 July 2024 118 2 View

Why do my media wells have a higher cell count than my antigen stimulated wells in ELISpot assay?

Hi, we are using IFNg-ELISpot assay on PBMC stimulated with Tet Tox and KLH. Unfortunately we are seeing higher spot counts in our media only wells and not sure why, could anyone please shed some...

08 November 2021 5,897 2 View

AID reader giving Cy3 images in fluorescent pink? How to change back to red?

We are running an IFNg/IL10 Fluorospot and our AID reader has changed the well colours during the calibration stage to a fluorescence pink (for FITC) when it should be red, CY3 is a light...

26 October 2021 9,834 0 View

Can we use bovine PPD on human PBMCs?

We are using PPD as a stimulant for proliferation assays (ELISpot and Thymidine) on human PBMCs and are having trouble finding more PPD. Has anyone used bovine PPD on human PBMCs in assay?

20 August 2021 6,184 1 View

Can you keep a working solution of detection antibodies for ELISpot assay in dPBS+FBS0.05% for a period of time or do they have to be used instantly?

Working with ELIspot Assays and want to know how long a working solution of detection antibodies in dPBS+0.05%FBS can last for, as we want to know if we can make up a large stock and instead of...

21 April 2021 2,569 3 View