Deepika Jain

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Questions related from Deepika Jain

Can we use a lysis buffer containing 0.1% Triton to lyse cells coated on Poly D lysine plates? Will it interfere protein estimation (BCA) post lysis?

The cells are genetically engineered and grown on PDL plates for a potency assay. My BCA is highly variable. I am wondering if triton disrupts the PDL coating, which then interferes in the BCA...

11 January 2024 5,258 1 View

Can we use a lysis buffer containing 0.1% Triton to lyse cells coated on Poly D lysine plates?

The cells are genetically engineered and grown on PDL plates for a potency assay. My BCA is highly variable. I am wondering if triton disrupts the PDL coating, which then interferes in the BCA...

11 January 2024 8,529 1 View