16 July 2019 1 5K Report

Can I treat balb/c mice with immunosuppressant and then use it for xenotransplant study? is it possible to do it this way? If not then why?

More Devendra Shukla's questions See All
Phytic acid limits bioavailability of micronutrients: Is challenge for biofortification?

As many researcher have confirmed that phytic acid content basically present in cereals and legumes crop that responsible for limiting bioavailability of micronutrients (Fe and Zn) in seeds. Lets...

31 January 2021 5,556 8 View

Can a compound have LogP value around 0.05 and LogD value around 4.02?

I understand that there might be huge differences between LogD and LogP for ionizable compounds. Normally, logD should be lower than logP for ionizable compounds. But can LogD value be...

05 January 2021 6,714 0 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

Biofilm Biofertilizers- New Generation biofertilizers?

Biofilm Biofertilizers-It is being applied to adhere the surface of soil and plant matrix containing consortia of various micro-organism that cut down chemical fertilizer. It is cost effective...

12 October 2020 4,883 13 View

Ethics in research publications?

In present scenario, lots of work were done by researchers and no doubt some of these have potential to change mindset of others that visible through benefiting farmers and other stakeholders....

09 October 2020 4,087 7 View

Can I please get some help with a suitable dissertation topic?

I am a PG student working on my dissertation related to effective document control using BIM. Also looking at alternative topic related to lean construction and BIM Can someone please help find...

14 September 2020 7,153 10 View

How to use commands in ansys workbench?

I am trying to input the material data in ansys mechanical with the help of command i have.This experimental data only consists of stress strain value of concrete cubes.I dont know where this code...

02 August 2020 7,769 5 View

How do I give glucose supplements to rabbits after Alloxan intra-peritoneal dose to prevent hypoglycemic shock?

Its study for alloxan-induced diabetes in the rabbit.

14 July 2020 2,869 1 View

How genetic algorithm can be used to find optimal weights and biases of neural network?

Assume a 3-2-1 (3 neurons in the input layer, 2 neurons in the hidden layer and 1 neuron in the output layer) neural network. How genetic algorithm can be used to find optimal weights and biases...

24 May 2020 1,778 2 View

Is there any evidence why COVID-19 cases started to increase in low-income countries after the cases started to decline in developed nations??

Is there any evidence why COVID-19 cases started to increase in low-income countries after the cases started to decline in developed nations? COVID-19 cases continuously now increasing in...

12 May 2020 4,884 8 View

Similar questions and discussions
How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close