Can I treat balb/c mice with immunosuppressant and then use it for xenotransplant study? is it possible to do it this way? If not then why?
https://www.nature.com/articles/cmi201639 Yes, cyclosporin + ketoconasol should work
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
"What is the most accurate method and model for evaluating the effective mechanical and viscoelastic properties due to the inclusion of multiple types of particles in a viscous material, and which...
16 June 2024 550 0 View
16 June 2024 9,139 1 View
I am trying to polish Cemented carbide sample for EBSD analysis. I have done the mechanical grinding and polishing up to 1/4um Dimond paste. At this level still I can see mechanical distortion....
07 May 2024 5,883 0 View
Is there a formula that I can use to calculate the power output using solar irradiance values?
01 May 2024 8,233 3 View
I am planning to measure the ethanol and acetaldehyde contents in the leaves and crowns of turfgrass that were subjected to varying durations of ice encasement. Does anyone have a protocol for...
07 April 2024 2,755 1 View
XRD has denoted its conversion into rGO but not finding its characteristic peaks (but only 2D band) in Raman is very confusing. Please suggest me something with references.
31 March 2024 2,362 3 View
Anyone please explain this
14 March 2024 6,976 2 View
I am interested in knowing if there is any sgRNA library designed against MDCK cell line which is a canine cell line. i believe it shares about 84% homology with human cell line. let me know if...
03 January 2024 5,148 0 View
In articles, researchers discuss about the charged ions in PBC structures using first principles investigation. How can we possibly provide charge to an atom in DFT during PBC investigation? e.g....
23 December 2023 1,380 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View