We're planning to sort live cells dissociated from mouse hippocampi according to nestin and GFAP reactivity. Does anybody have any experience with performing intracellular staining without fixation?
Thank you.
I am using VASP and vaspkit to plot Local Density of States. I am trying to see the LDOS difference in different position of the structure. How to plot LDOS against distance (Ang) instead of...
10 June 2024 9,014 1 View
Hi, I am using VASP to simulate interface of 2 block material slabs. After bringing them together I can get the stress tensor for the whole simulation box from OUTCAR. However, is there any way to...
23 May 2024 3,500 1 View
Is there any software that can take atomic positions as input (e.g. VASP POSCAR file) and give me the output of Wyckoff positions? I am trying to find vacancies of some specific Wyckoff positions...
19 May 2024 7,866 3 View
In the optical examples of GEANT4, specifically in the cerenkov.mac file where the LXe geometry is defined, is it possible to change the geometry and material of the scintillator and PMTs from LXe...
08 May 2024 7,236 0 View
Hello, I have isolated extracellular vesicles from a cell culture using the Exosome isolation kit. However, the western blot results did not show up because the concentration was only 0.2 ug/ul. I...
15 February 2024 8,378 4 View
In the SSR method, I used the same pair of primers (forward and reverse) in two different individuals, but I observed different band patterns in PCR.
05 February 2024 3,438 2 View
Genetic Engineering. Assuming that there were no errors during the process.
03 February 2024 2,832 0 View
Genetic Engineering. Sequence. mRNA sequence.
03 February 2024 1,375 2 View
Genetic Engineering. Markers.
03 February 2024 4,012 0 View
Genome library. Restriction Enzymes. Genetic engineering.
03 February 2024 5,429 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
“Yerkes (1912) trained an earthworm to choose one arm of a T-maze, using electric shock as punishment for error and the moist burrow as reward for correct choice. The habit was acquired in twenty...
31 July 2024 8,809 0 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View