How to find the air side pressure drop in a spiral fin tube bundle cross flow heat exchanger?

How to find the air side pressure drop in a spiral fin cross flow heat exchanger? The pressure difference at the inlet and outlet is very low compared to the experimental results, which I...

18 February 2021 1,109 2 View

Which sources you would suggest for finding unpublished research articles in order to include in meta-analysis?

Which sources you would suggest for finding any sort of unpublished research articles (e.g., pre-prints, rejected manuscripts, articles from grey-area, dissertations) in order to include in a...

09 January 2021 7,269 7 View

What is the reason for observing two bands of beta-actin after lipofectamine transfection of keratinocytes cells?

25 December 2020 8,668 3 View

Does anybody knows anything about INFORMATICA JOURNAL (SCI expanded), which publishe article at a cost of 423 US/or equivalent bitcoin dollars?

Does anybody knows anything about INFORMATICA JOURNAL (SCI expanded), which publishes an article at a cost of 423 US/or equivalent bitcoin? Is this journal fake or true?

15 December 2020 8,985 2 View

What kind of a relation is there between the cement hydration and the concrete properties?

How are the concrete properties changed by the differences in hydration?

24 May 2020 4,009 10 View

Blood or tissue sample for cytokine assay?

Hello all, I would like ask whether blood or colon tissue could be a better option to analyze TNF-alpha, IL-1B, IL-10, IL-6 levels in mice colitis model. Also which method would be a better...

08 December 2019 5,442 3 View

Can you share the names of research centers which gather the video-recordings of adult psychotherapy sessions?

Dear colleagues, I would like to conduct my independent research on psychotherapy processes of individuals with pathological narcissism. I would really appreciate if you can share your contacts...

10 November 2019 6,193 2 View

Does Kapa genotyping kit suitable for TA Cloning ?

Hello, I am extracting mouse DNAs and performing PCRs through KAPA 2G Fast hotstart genotyping kit. Before I use the pcr products for TA Cloning (invitrogen), I am purifying PCR products through...

14 October 2019 2,984 2 View

I cannot detect Parp protein after total protein isolation?

I cannot detect Parp protein after total protein isolation Hello everyone; I cannot detect the Parp protein after total protein isolation. I can see my cells die morphologically after a novel...

18 July 2019 2,593 2 View

At what stage should I use the DNAse I reaction protocol?

Protocol content (NEB DNAse I reaction Protocol) : RNA: 10ug DNAse reaction buffer 1X 10ul DNAse (Rnase free) 1ul Nuclease free water 100 ul My question is; if I use this protocol after isolating...

11 January 2019 1,249 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View